Today's important lesson in science

[danaoshee]

Keeping your polymerase solution cold is good.
Putting so much dry ice in your ice bucket (to keep the water-ice frozen all day) that your polymerase solution FREEZES SOLID is bad.

This lesson brought to you by my coworker who really should know better.

On the bright side, now I don't have to stay late...:)

Comments

[shalyndra]

holy crap, I didn't know that was possible. Did it wreck the polymerase?

[danaoshee]

Well, it's not the raw polymerase/glycol solution, it was the binding mix with buffer and template in already.
And yes, it killed the experiment for the day, and it would have required another 2 hour incubation to get it prepped again..

So his experiment was canceled for the day and I didn't have to stay late running it.

[grandmoffdavid]

I feel your pain--as though a thousand wronged lab partners cried out, and then were suddenly silenced...

[danaoshee]

Also, this example of stunning intelligence is a SENIOR SCIENTIST getting paid way more then me. argh.

[davio]

So, the moral of this story is one should be cool but not frigid when dealing with poly?