Tumor specific cytotoxicity assay. The CTL activity was measured by a modified flow cytometric carboxy-fluorescein succinimidyl ester/7-amino actinomycin D (7-AAD) cytotoxicity assay. Briefly, the splenocytes isolated from treated mice on day 26 served as the effector after 5 days' stimulation with irradiated (200 Gy) 4T1 cells (30:1) in the
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