How not to get cancer...

[girlblue_77]

Since I do breast cancer research, and am constantly attending talks about the environment and women's health and cancer in general, I thought I would share with you a few I've learned about cancer prevention. Cancer is tragically common, and there are simple things we can do to lower our risks. Also, some of you are likely to have already had

Comments

[budgie_]

I heart broccoli. And green beans, too. :)

[donnalee_kiss]

Thank you dear for providing a great memory!

[trystesse]

hey - thanx! that's a really nice post! I'd add - try not to worry about it too much! Coz a stressed brain can bring on illnesses...

Your transwell assay question

[anonymous]

Hello! I don't have a livejournal account and the scientists' live journal won't allow anonymous comments...

To answer your question about the 1%BSA for the transwell migration assay:

We often use BSA in cell culture when cells need to be cultured in the absence of "regular" serum (FBS). If you were using FBS/serum as your chemoattractant in the assay, or not using serum at all, then you may want to use BSA to keep your cells alive long enough for the assay to be completed. However, it sounds like you're not using serum as a chemoattractant, but rather some other compound (I've done this before too). In that case, my personal opinion would be that the BSA would be irrelevant as long as you had seeded your cells in regular serum. If you have any other protocol questions, let me know!

Good luck!
Lisa (LMPrivet@umich.edu)

Re: Your transwell assay question

[girlblue_77]

THANK YOU.

You answered my question perfectly.

Very, very helpful! :)

[livewire24]

interesting to note that u can turn orange due to excessive consumption of carrots...dint see that one coming!!!
oh and the info on breast cancer was good too ;)

sangeetha