haha, my friggin plasmid is finally right and i can FINALLY transvect into my little Trypanosome buddies!!! YES, that only took like 5 months of crap and my whole fuckin summer to do. Funny thing is, i highly doubt the RNAi is going to work anyway, cuz i guess Sandi tried it in 3 different vectors and couldnt get the knockdown. So i know no one
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