Yarrrr. Hooray! 'Twil be a right noble lab report indeed, matey.
So ... what be up with the band around 375 bp in the purportedly plasmid sans primer column? The cross contamination between the cDNA plasmids and the primers be obvious indeed, but it doesn't explain that (at least not to this lubber). Contamination from another source? Damage to the plasmids? It doesn't show up in the plasmid/primer column, so it would have happened after you took that sample. Obviously your gel electrophoresis technique is flawless, so that's not a source of error. Arrr.
Were ye not goin'ta run the cursed chicken plasmids one final time?
I reckon' they'd catch on iffin ye tried to bring a thermocycler and gel rack home wit' ye? &sigh; Alas, I be only half a pirate-geneticist. (Though avast, I'd just as soon ye'd leave the EtBr behind when a-boardin' me bonny vessel 'ere.)
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So ... what be up with the band around 375 bp in the purportedly plasmid sans primer column? The cross contamination between the cDNA plasmids and the primers be obvious indeed, but it doesn't explain that (at least not to this lubber). Contamination from another source? Damage to the plasmids? It doesn't show up in the plasmid/primer column, so it would have happened after you took that sample. Obviously your gel electrophoresis technique is flawless, so that's not a source of error. Arrr.
Were ye not goin'ta run the cursed chicken plasmids one final time?
I reckon' they'd catch on iffin ye tried to bring a thermocycler and gel rack home wit' ye? &sigh; Alas, I be only half a pirate-geneticist. (Though avast, I'd just as soon ye'd leave the EtBr behind when a-boardin' me bonny vessel 'ere.)
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