Comments | selkiss: Lab victory!

selkiss

Lab victory!

May 03, 2007 15:35

So yesterday my standard arrived. Today I did an assay with it.

My standard curve has an r squared value of 1.

1!

Usually you get 0.9 something I have never ever had such a linear standard curve.

Joy!

I sure hope some of the people reading this understand my excitement
*listens*
*hears crickets*
*sees a tumbleweed*

*cuss*

Comments 17

aallecks May 3 2007, 06:40:56 UTC

Congrats!! :)

selkiss May 3 2007, 23:41:08 UTC

Hehe thanks.

I actually can claim some credit for it working because while the standard was bought, I did the dilutions and added reagents. *dances*

dungeoncandy May 3 2007, 06:57:28 UTC

omg i actually did get what u said. old skool maths missy. but yes.

well done on your curve.

ps. LINEAR STANDARD IN MY PANTS!

selkiss May 3 2007, 23:43:03 UTC

Yay! It's always called "curve" despite it being linear in some cases.

Oh dear.

dungeoncandy May 4 2007, 05:55:14 UTC

thats what she said!

pippilicious May 4 2007, 01:48:53 UTC

p.s. Your Mum's a linear standard.

Thread 5

pippilicious May 4 2007, 01:48:30 UTC

Isn't it satisfying?! :D

yay you!

selkiss May 4 2007, 02:02:29 UTC

It was more than satisfying. I kinda did a doubletake then showed anyone I could find. Even though it should technically be 1, I think it is probably rare for that to happen due to slight fluctuations in everything.

I am satified by my serial dilution technique though as the curve would not have been good if I failed at that.

Now alls I gotta do is keep getting perfectly linear curves and find a way to throw it into my thesis somehow...or not.

neva9257 May 4 2007, 03:36:49 UTC

It is satisfying, no? I haven't got *1*, but i've pulled .99995 before, which i almost as good. Here crickets no, more, for i shall whoop with joy alongside.

selkiss May 4 2007, 04:00:33 UTC

I totally beat you! This is cause for celebration!
But hooray for 2.5 (or possibly 3) people understanding my elation.

the_m0nster May 4 2007, 10:19:30 UTC

You can explain some time and I will understand.
For the nonce, I will smile warmly at your esoteric success! At least I understand that!
*EEEEEEE*
Sorry, that was my ludicrous smile. I suppose the warm and gentle one doesn't make any sound.

selkiss May 4 2007, 23:09:51 UTC

I can imagine the ludicrous smile and I think I like it better.
Hehe.

the_m0nster May 6 2007, 09:42:04 UTC

I enjoy it!