Is it safe to assume that the transfection efficiency of firefly gene and sea pansy gene are independent but probably similar (that the chance of cell q taking in the firefly gene is independent of the chance of cell q taking in the sea pansy gene, but if about 80 out of 100 cells take in firefly gene using whatever method you are using then about 80 out of 100 cells take in sea pansy gene)?
I'm not sure if I need that, but the question occurred as I began processing it in my head...
Yes - for the purpose of the experiment it is assumed that if cell q takes in the sea pansy gene, it also takes in the firefly gene, and if it doesn't take in sea pansy, it also doesn't take in firefly.
In between assignments at work for the moment, he turns to others' work...
anonymous
July 19 2004, 07:13:35 UTC
- Shouldn't C(n(s)) = C(s) and C(n(f)) = C(f), since they're measurements from cells that have had the same things done to them, namely transfection with both genes and no shRNA? In fact, I would expect that there's exactly one control mush. That oughta simplify things a bit
( ... )
Re: In between assignments at work for the moment, he turns to others' work...silvergirl354July 19 2004, 16:58:10 UTC
Thanks Because it is bio, yes, all those things should equal each other, but don't. I ended up getting something similar to what you ended up with. I hashed it out at work and ... yeah, everyone agrees it's a mess, so we'll see what we can do with it.
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I'm not sure if I need that, but the question occurred as I began processing it in my head...
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Because it is bio, yes, all those things should equal each other, but don't. I ended up getting something similar to what you ended up with. I hashed it out at work and ... yeah, everyone agrees it's a mess, so we'll see what we can do with it.
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