Comments | toxins: Experimental...Why? Why?

toxins

Experimental...Why? Why?

Jul 21, 2005 09:49

Why is it so difficult in immunohistochemistry to find the proper secondary antibody (with conjugate) that ( Read more... )

academic

Comments 18

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Hmmm... toxins July 21 2005, 22:23:16 UTC

Not too sure about that; maybe he is just trying to tell me to use Alexa fluor instead of Texas Red.

word,
toxins

loucheroo July 21 2005, 14:10:21 UTC

ive been asking myself the same thing for years.....

Hmmm... toxins July 21 2005, 22:23:41 UTC

I bet you have.

word,
toxins

mustardbear July 21 2005, 17:41:21 UTC

I think it's because they're hiding.

Hmmm... toxins July 21 2005, 22:24:29 UTC

Obviously this is the answer that is most close to true. They actually hide in the background signal. Wow. Are you a scientist?

word,
toxins

Re: Hmmm... mustardbear July 22 2005, 12:01:40 UTC

No, but I did stay at a Holiday Inn Express last night!

Re: Hmmm... toxins July 22 2005, 13:35:29 UTC

OMG...I've said this before. Dude, I laughed so hard you just made milk shoot out my nose. lol.

BTW, how is the new pad?

word,
toxins

Thread 6

twilyght_zone July 21 2005, 20:00:01 UTC

Obviously I don't have the answers, however, I am certain that if you keep at it,YOU will eventually find those answers.

Love & laughter
me

Hmmm... toxins July 21 2005, 22:25:40 UTC

Thanx, I'll do my best. I already have a few ideas (try different secondary, use different blocking agent, excitation at a different frequency, use antibody with different emission frequency). Just not sure which to use first.

word,
toxins

angryhippie July 23 2005, 00:12:26 UTC

um... you lost me at immunohistochemistry. but good luck ;-)

Peace toxins July 25 2005, 02:19:33 UTC

Thanx man.

word,
toxins