May 25, 2005 20:20
After spending the whole day on a competent cell prep, the styrofoam box I carrying them in broke while I was coming down the outside staircase, casting the neat little frozen eppendorfs over a vast area of questionable sterility. Well, I can always do it again...
Anyone care to remind me why I wanted to spend the rest of my life doing this?
Leave a comment
Comments 1
But, you know, if you just wiped down the outside of the tubes with ethanol, you'd probably be ok - epps are fairly contamination-proof, and you'll (presumably? unless you are using some special sort of selection?) be selecting your transformants with antibiotics anyway. No help now, I realise, but maybe for next time.
Reply
Leave a comment